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Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta Indica A. Juss.) Terhadap Struktur Histologi Intestinum Tenue Mencit (Mus Musculus L.) Jantan

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ABSTRAK

Daun Mimba (Azadirachta indica A. Juss.) mengandung bahan aktif seperti azadirachtin, nimbin, nimbidin, meliantriol, tanin dan salannin yang biasa digunakan sebagai obat tradisional. Penelitian ini bertujuan untuk menguji pengaruh ekstrak etanol daun mimba (Azadirachta indica A.Juss) terhadap struktur histologi intestinum tenue mencit (Mus musculus L.) Jantan. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 2 perlakuan yaitu K (akuades) dan P (Perlakuan ekstrak etanol daun Mimba dengan dosis 14 mg/kg BB/hari) masing-masing dengan 15 kali ulangan. Perlakuan diberikan secara oral dengan volume 0,2 mL selama 21 hari. Pemberian pakan dan minum dilakukan secara ad libitum. Intestinum tenue pada bagian ileum diisolasi, ditimbang dan dibuat sediaan histologis menggunakan metode parafin dengan tebal sayatan 5 μm dan pewarnaan Hematoksilin dan Eosin (H&E). Pengukuran diameter, tinggi vili dan tebal mukosa intestinum tenue pada bagian ileum dilakukan pada 2 sayatan di setiap sediaan histologi intestinum tenue pada bagian ileum. Data dianalisis menggunakan uji t pada taraf kepercayaan 95%. Hasil analisis menunjukkan paparan bahan uji memberikan tidak berpengaruh nyata (p>0,05) terhadap diameter, tinggi vili dan tebal mukosa intestinum tenue pada bagian ileum. Hasil analisis terhadap bobot badan dan bobot saluran digesti juga tidak berpengaruh nyata (p>0,05). Penelitian ini dapat disimpulkan bahwa pemberian ekstrak etanol daun Mimba selama 21 hari pada mencit jantan aman untuk organ intestinum tenue di bagian ileum.
Kata kunci: Azadirachta indica, Ileum, Intestinum Tenue
 
ABSTRACT

Neem leaves (Azadirachta indica A. Juss.) contain some active ingredients such as azadirachtin, nimbine, nimbidine, meliantriol, tannin and salannin which are commonly used as traditional medicine. The aim of this study was to examine the effect of ethanol extract of the leaves of Neem (Azadirachta indica A.Juss) on the tenue instestines histological structure of male mice (Mus musculus L.). This study used a Completely Randomized Design (CRD) consisting of 2 treated groups, namely K (control group, treated with auqadest) and P (Treated with ethanolic extract of neem leaves at a dose of 14 mg/kg BW / day) each with 15 replications. Treatment was given orally with a volume of 0.2 mL for 21 days. Feeding and drinking are done in ad libitum. The mice were sacrificed at the 22nd days by chloroform anesthezied. The tenue intestines was isolated and weighed. The ileacal part of tenue intestines were isolated and made for histological preparations using paraffin method with 5 μm in thickness and Hematoxylin-Eosin (HE) staining. The measurement of diameter, villi height and mucosal thickness of the ileacal tenue intestines was performed at every 2 sections in each histological slides. Data were analyzed using the t-test at the 95% confidence level. The results of the analysis showed that exposure to the ethanolic neem leaves extract gave no significant effect (p> 0.05) on the diameter, height of villi and mucous thickness of the ileacal tenue intestines. The body weight and digestive tract weight was had no significant effect (p> 0.05) also. It could be concluded that the administration of ethanolic neem leaves extract 21 days in male mice was safe for the ileacal tenue intestines structure.
Keywords: Azadirachta indica, ileacal, tenue intestines

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Ketersediaan

1323B19III

1323 B 19-ii

Perpustakaan FSM Undip (Referensi)

Tersedia

Informasi Detail

Judul Seri

BIOLOGI

No. Panggil

1323 B 19-ii

Penerbit

: ., 2019

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

519.6

Tipe Isi

-

Tipe Media

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Tipe Pembawa

-

Edisi

-

Subjek

-

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Iffah Hikmatul Azizy

Versi lain/terkait

Lampiran Berkas

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