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Struktur Hepar Dan Rasio Bobot Hepar Terhadap Bobot Tubuh Mencit (Mus musculus L.) Jantan Setelah Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A.Juss)

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ABSTRAK

Penelitian ini bertujuan untuk mengetahui struktur hepar dan rasio bobot hepar terhadap bobot tubuh mencit (Mus musculus L) jantan setelah pemberian ekstrak etanol daun mimba (Azadirachta indica A.Juss). Penelitian ini menggunakan desain Rancangan Acak Lengkap (RAL) dengan 2 kelompok perlakuan yaitu Kontrol (Akuades) dan Perlakuan (Pemberian ekstrak etanol daun mimba dengan dosis 14 mg/KgBB/hari) masing-masing dengan 15 ulangan. Perlakuan diberikan secara oral dengan volume 0,2 ml selama 21 hari. Hepar diisolasi, ditimbang dan dibuat preparat menggunakan metode paraffin dengan ketebalan 5 µm pewarnaan Hematoksilin Eosin (HE). Pengamatan struktur hepar dan pengukuran diameter hepatosit dilakukan dalam 4 bidang pandang, setiap bidang pandang diambil 3 lobulus dan setiap lobulus diambil 4 hepatosit. Bobot hepar dan bobot tubuh digunakan untuk menghitung rasio bobot hepar terhadap bobot tubuh atau disebut dengan nilai Hepatosomatik Indek (HSI). Data dianalisi dengan uji t dengan taraf kepercayaan 95%. Hasil analisis menunjukan adanya pengaruh nyata terhadap diameter hepatosit mencit (Mus musculus) jantan setelah pemberian ekstak etanol daun mimba (Azadirachta Indica A.Juss) tetapi menunjukan pengaruh tidak nyata terhadap bobot tubuh, bobot hepar dan nilai Hepatosomatik indek (HSI). Hal ini dapat disimpulkan bahwa pemberian ekstrak etanol daun mimba sebagai obat tradisional tidak mempengaruhi struktur hepar dan rasio bobot hepar terhadap bobot tubuh mencit (Mus musculus) jantan bila diberikan pada dosis yang tepat.

Kata kunci : Daun Mimba, Hepar, Diameter Hepatosit dan HSI.

ABSTRACT

The aim of this study was to determine the liver structure and the ratio of liver weight to the body weight of male mice (Mus musculus L) after the administration of ethanolic of neem leaves (Azadirachta indica A.Juss) extract. This study using a Completely Randomized Design with 2 treated groups, namely control (treated with aquadest) and treated group (giving ethanolic neem leaves extract at a dose of 14 mg /BodyWeight/ day) each with 15 replications. Treatment was given orally with a volume of 0.2 ml for 21 days. At the 22nd days, the liver was isolated, weighed and contiuned by histologically prepared with paraffin method, using HE staining with 5 μm in thickness. Observation of the liver structure and measurement of hepatocyte diameter were carried out in every 4 fields of the organs, 3 lobules were taken in each field and 4 hepatocytes were taken from each lobule. The liver weight and body weight were used to calculate the ratio of liver weight to body weight or called the Hepatosomatic Index value (HSI). Data was analyzed by T Test with a confidence level of 95%. The results showed a significant effect on the hepatocyte diameter of male mice (Mus musculus) but showed no significant effect on body weight, liver weight and Hepatosomatic index value (HSI). It could be concluded that treated with the ethanolic neem leaves extract during 22 days was safe to the male mice liver structure. It can be concluded that the administration of ethanolic of neem leaves (Azadirachta indica A.Juss) extract as traditional medicine did not effect the liver structure and the ratio of liver weight to the body weight of male mice (Mus musculus L) if given at the right dose.

Keyword : Neem Leaves, Liver, Hepatocyte Diameter and HSI.

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Ketersediaan

1307B19III

1307 B 19-ii

Perpustakaan FSM Undip (Referensi)

Tersedia

Informasi Detail

Judul Seri

BIOLOGI

No. Panggil

1307 B 19-ii

Penerbit

: ., 2019

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

519.5

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

-

Subjek

-

Info Detail Spesifik

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Pernyataan Tanggungjawab

Ratih Nur Fitriani

Versi lain/terkait

Lampiran Berkas

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