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Pengaruh Pemberian Ekstrak Etanol Daun Mimba (Azadirachta Indica A.Juss) Terhadap Bobot Uterus Dan Tebal Endometrium Mencit (Mus Musculus L.)

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ABSTRAK

Daun Mimba (Azadirachta indica A. Juss.) mengandung bahan aktif seperti flavonoid, triterpenoid, alkaloid, tanin dan saponin yang berpotensi sebagai senyawa antifertilitas. Penelitian ini bertujuan untuk menguji pengaruh ekstrak etanol daun mimba (Azadirachta indica A.Juss) terhadap berat uterus dan tebal endometrium mencit (Mus musculus L.). Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 5 perlakuan yaitu K- (Akuades), K+ (Pil Kontrasepsi), P1, P2 dan P3 (Perlakuan ekstrak etanol daun Mimba dengan dosis 8,4; 11,2 dan 14 mg/kg BB/hari) masing-masing dengan 5 ulangan. Perlakuan diberikan secara oral dengan volume 0,2 mL selama 21 hari. Pemberian pakan dan minum dilakukan secara ad libitum. Uterus diisolasi, ditimbang dan dibuat sediaan histologis menggunakan metode paraffin dengan tebal sayatan 5 µm dan pewarnaan Hematoksilin Eosin (HE). Pengukuran tebal endometrium dilakukan pada 6 sayatan di setiap sediaan histologi uterus. Data dianalisis menggunakan Analysis of Variance (ANOVA) pada taraf kepercayaan 95% dan uji lanjut DMRT. Hasil analisis menunjukkan paparan bahan uji memberikan pengaruh yang nyata (p0,05) terhadap bobot uterus dan bobot total organ reproduksi. Penelitian ini dapat disimpulkan bahwa pemberian ekstrak etanol daun Mimba selama 21 hari dapat memengaruhi fertilitas dengan cara menurunkan tebal endometrium.
Kata kunci: Antifertilitas, Reproduksi, Histologi.

ABSTRACT

Neem leaves (Azadirachta indica A. Juss.) contain some active ingredients such as flavonoids, triterpenoids, alkaloids, tannins and saponins compounds that have potencies antifertility. The aim of this studywas to examine the effect of neem leaf ethanol extract (Azadirachta indica A.Juss) toward weight of uterine and endometrial thickness mice (Mus musculus L.). This study was used a Completely Randomized Design concist of 5 treatments group with 4 replications, Namely K-(Aquadest), K+(Contaceptive Pills), P1, P2 and P3(each were treated by neem leaf ethanol extract with dosage of 8,4; 11,2 and P3 mg/kgBW/day). The treatment was administered orally with 0,2 mL every morning for 21 days. The feeding and drinking in ad libitum. The uterus were isolated, weighed and histologically processed using paraffin method with a 5 μm incision thickness and Hematoxylin Eosin (HE) staining. Measurements of endometrial thickness were performed at 6 incisions in each uterine histologically processed. The data was analyzed using Analysis of Variance (ANOVA) at 95% confidence level and continued by DMRT test. The results of the analysis showed that exposure to the test material had a significant effect (p 0.05) on uterine weight and total weight of the reproductive organs. This study concluded that the administration of ethanol extract of Mimba leaves for 21 days can affect fertility by reducing endometrial thickness.
Keywords:Antifertility, reproduction, histology

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Ketersediaan

1290B19I

1290 B 19-i

Perpustakaan FSM Undip (Referensi)

Tersedia

Informasi Detail

Judul Seri

BIOLOGI

No. Panggil

1290 B 19-i

Penerbit

: ., 2019

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

005.7565

Tipe Isi

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Tipe Media

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Tipe Pembawa

-

Edisi

-

Subjek

-

Info Detail Spesifik

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Pernyataan Tanggungjawab

Anis Alfiyanti

Versi lain/terkait

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