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Komunitas Bakteri Rhizosfer Teh Melalui Aplikasi Biomunizer (Chryseobacterium sp. Dan Alcaligenes sp.) Dengan Metode Terminal Restriction Fragment Length Polymorphism (T-RFLP) 579 AVI k

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ABSTRAK

Jenis bakteri yang berasal dari komunitas rhizosfer sebenarnya sudah banyak
dikembangkan sebagai bioimunizer namun konsistensinya terkadang masih
diragukan, karenanya dibutuhkan bakteri indigenous yang berasosiasi dengan
rhizosfer tanaman. Penelitian ini bertujuan untuk memberikan informasi mengenai
komunitas bakteri rhizosfer tanaman teh yang pada penelitian sebelumnya telah
diinfeksikan E.vexans Massee lalu diberikan bioimunizer serta memeriksa
keberadaan Chryseobacterium sp. dan Alcaligenes sp. sebagai bentuk konsistensi
pengaplikasian bioimunizer pada tanaman teh. Komunitas bakteri pada sampel
kontrol melalui pengkulturan diperoleh Bacillus sp. (51,91%), Acidobacteria
bacterium (39,42%) dan Actinobacteria sp. (8,66%) sedangkan melalui metagenom
diperoleh Gemmatimonas aurantiaca (5,80%), Bacillus sp. (42,55%),
Acidobacteria bacterium (23,45%) dan Actinobacteria sp. (28,20%). Komunitas
bakteri pada sampel perlakuan melalui pengkulturan diperoleh Gemmatimonas
aurantiaca (3,58%), Bacillus sp. (30,76%), Pseudomonas sp. (5,55%)
Acidobacteria bacterium (13,94%) dan Actinobacteria sp. (46,16%) sedangkan
melalui metagenom ditemukan Bacillus sp. (10,66%), Acidobacteria bacterium
(4,22%), Actinobacteria sp. (5,48%), Uncultured bacterium (1,49%), Alcaligenes
sp (36,95%) serta Chryseobacterium sp. (46.82%). Keberadaan Alcaligenes sp. dan
Chryseobacterium sp. menunjukkan konsistensi komposisi bioimunizer yang
diaplikasikan. Komunitas bakteri pada metagenom memiliki tingkat
keanekeragaman dan kemerataan (evenness) yang lebih tinggi dibandingkan
dengan pendekatan pengkulturan.
Kata kunci: komunitas bakteri, T-RFLP, Chryseobacterium sp., Alcaligenes sp.

ABSTRACT

Types of bacteria from the rhizosphere communities actually have been developed
as bioimunizer but sometimes consistency is still in doubt, would require
indigenous bacteria associated with rhizosphere of plants. This study aims to
provide information on the tea plant rhizosphere bacterial communities which in
previous studies has been infected with E. vexans Massee and given bioimunizer as
well as the checking existence Chryseobacterium sp. and Alcaligenes sp. as a form
of consistency in the application of tea plant bioimunizer. Communities of bacteria
on control samples obtained through culturing Bacillus sp. (51.91%), Acidobacteria
bacterium (39.42%) and Actinobacteria sp. (8.66%) while through metagenom
obtained Gemmatimonas aurantiaca (5.80%), Bacillus sp. (42.55%), Acidobacteria
bacterium (23.45%) and Actinobacteria sp. (28.20%). Communities of bacteria in
the samples treated by culturing obtained Gemmatimonas aurantiaca (3.58%),
Bacillus sp. (30.76%), Pseudomonas sp. (5.55%) Acidobacteria bacterium
(13.94%) and Actinobacteria sp. (46.16%) while through metagenom found
Bacillus sp. (10.66%), Acidobacteria bacterium (4.22%), Actinobacteria sp.
(5.48%), Uncultured bacterium (1.49%), Alcaligenes sp. (36.95%) and
Chryseobacterium sp. (46.82%). The existence of Alcaligenes sp. and
Chryseobacterium sp. show consistency bioimunizer composition is applied.
Communities of bacteria on metagenom have diversity and evenness level higher
than the culturing approach.
Keywords: bacterial communities, T-RFLP, Chryseobacterium sp., Alcaligenes sp.

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Ketersediaan

1048B17I

1048 B 17

Perpustakaan FSM Undip (Referensi)

Tersedia

Informasi Detail

Judul Seri

BIOLOGI

No. Panggil

1048 B 17

Penerbit

: ., 2016

Deskripsi Fisik

-

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

1446

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

-

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Elin Safitri Aviani

Versi lain/terkait

Lampiran Berkas

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