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Efektivitas Pakan dengan Suplementasi Daging Ikan Gabus (Channa striata) terhadap Perbaikan Histologi Duodenum Tikus Wistar setelah Mengalami Pengondisian Stres Fisiologis 571.1 GUL e
ABSTRAK
Stresakibatkekurangandandefisiensinutrisipakan yang
diikutiaktivitasberlebihanmerupakanfaktorpemicuterjadinyagangguanstrukturdanf
ungsi duodenum.Organ pada duodenum
memilikikemampuanregenerasisecaraperiodiksetelahmengalamigangguanakibatstr
es fisiologis.Kandungannutrisipakanmenentukan proses regenerasiorgan
duodenum usushalus. Penelitianinidilakukanuntukmengkaji,
menganalisisdanmenentukankonsentrasi daging ikan gabus untuk peningkatan
diameter, panjang vili, ketebalan lapisan mukosa, submukosa dan muskularis
duodenum tikusWistarsetelahpengondisianstress
fisiologis.Penelitianinimenggunakan 20 ekortikusWistarjantanterdiriatas 5
perlakuandengan 4 kali ulangan.PerlakuanpadapenelitianiniterdiriatasP0: kontrol,
hewanuji yang dikondisikanstres 6 hari yang
diikutipemberianpakantanpasuplementasidagingikangabus; P1, P2, P3,
danP4adalahhewanuji yang dikondisikanstresselama 6
haridandiberipakandengansuplementasidagingikangabusselama 14 hari, berturutturut 5%, 10%, 15%, dan 20% dariwaktusetelahpengondisianstres.
PenelitianinimenggunakanRancanganAcakLengkap (RAL).Parameter yang
diukurpadapenelitianiniadalah diameter, panjangvili, danketebalanlapisanmukosa,
submukosa, danmuskularis duodenum usushalus.Data yang
diperolehdianalisismenggunakanAnalysis of Variance (ANOVA)
dandilanjutkandenganuji Duncan dengantarafsignifikansi 95%.Hasil
penelitianiniadalahpemberian pakan daging ikan gabus dapat memperbaiki stuktur
histologis duodenum setelah pengondisian stres dan
pemberianpakandengansuplementasidagingikangabusdengankonsentrasi 15%
memberipengaruhpadapeningkatan diameter, panjangvili,
danketebalanlapisanmukosa, submukosa, danmuskularis duodenum, berturut-turut
2520 µm, 576,34 µm, 506 µm, 123,34 µm, dan 72,34 µm masingmasinglebihtinggi 23,43%, 46,37%, 45,26%, 50,31%, dan 22,95%
dibandingkontrol. Kesimpulan penelitian ini
suplementasidagingikangabusdalampakandapatdigunakanuntukmemperbaikistrukt
urdanfungsi duodenum pascastres.
Kata Kunci: stres, suplemen, duodenum, ikangabus, Channastriata, tikusWistar
ABSTRACT
Histologic Improvement after Experiencing Conditioning Physiological
Stress, Under the Guidance ofSunarno and Siti Muflichatun Mardiati.
Stress due to lack of feed and nutritional deficiencies that followed excessive
activity is a trigger factor disturbance structure and function of the duodenum.
Organ in the duodenum has the ability to regenerate periodically after
experiencing problems due to physiological stress. Nutrient content of feed
determine organ regeneration process duodenum of the small intestine. This study
was conducted to assess, analyze and determine the concentration of fish meat
cork to increase in diameter, villous length, the thickness of the layer of the
mucosa, submucosa and muscularis duodenum of Wistar rats after conditioning
the physiological stress.This study used 20 male Wistar rats consisted of 5
treatments with 4 replications. The treatment in this study consisted of P0: control,
test animals were conditioned stress followed six days of feeding without
supplementation with fish meat cork; P1, P2, P3, and P4 are test animals that were
conditioned stress for 6 days and fed with supplementation of fish meat cork for
14 days, respectively 5%, 10%, 15%, and 20% of the time after conditioning
stress. This study uses a completely randomized design (CRD). The parameters
measured in this study is the diameter, villous length and thickness of the layers of
mucosa, submucosa, and muscularis duodenum of the small intestine. Data were
analyzed using Analysis of Variance (ANOVA) followed by Duncan test with
95% significance level. The results of this study are feeding the fish meat cork can
improve the structure of histologic duodenum after stress conditioning and
feeding with supplementation of fish meat cork with a concentration of 15%
giving effect to increase in diameter, the length of villi, and the thickness of the
lining mucosa, submucosa, and muscularis duodenum, respectively -turut 2520
μm, 576.34 μm, 506 μm, 123.34 μm and 72.34 μm respectively higher 23.43%,
46.37%, 45.26%, 50.31%, and 22 , 95% compared to controls. It concluded
supplementation with fish meat cork in feed can be used to improve the structure
and function of post-stress duodenum.
Keywords: stress, supplements, duodenum, cork fish, Channa striata, Wistar
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