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Efek Audible Sound Terhadap Pertumbuhan, Kadar Lipid, dan Kadar Klorofil dari Chlorella vulgaris
This study analyzed the effect of audible sound on growth rate, lipid content,
and chlorophyll content in Chlorella vulgaris, using a melody entitled “Blues for
Elle” with exposure duration and certain frequency variations, namely 0, 50, 440,
and 1000 Hz. This study aims to determine the growth rate of C. vulgaris cultivated
with exposure to audible sound at certain frequency variations and determine the
value of lipid and chlorophyll levels in C. vulgaris under the same conditions. The
results showed that sound frequency significantly affected the growth, chlorophyll
production, and lipid content of C. vulgaris. Increased proliferation of C. vulgaris
occurred at frequencies of 50 Hz and 440 Hz with growth rates of 0.065/day and
0.076/day, respectively. In contrast, frequencies of 1000 Hz and 0 Hz decreased the
growth rate to 0.033/day and 0.03/day. Audible sound also caused an increase in
total chlorophyll production, with the highest level of 51.88 μg/mL at a frequency
of 1000 Hz. However, total lipid levels decreased with increasing sound frequency,
which amounted to 2.35% of dry biomass at 1000 Hz, due to oxidative stress. These
results indicate that specific sound frequencies can enhance growth and chlorophyll
synthesis, although higher frequencies may induce oxidative stress that inhibits
lipid biosynthesis in C. vulgaris. This study contributes to the development of
applications in biotechnology and bioenergy by optimizing the growth conditions
for C. vulgaris.
Keywords: audible sound, biosynthesis, lipid, microalgae, oxidative stress
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