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Molecular Identification of Mudskipper Fish from Bipolo and Oesapa, East Nusa Tenggara, Indonesia

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ndonesia hosts a variety of unique marine and terrestrial species, including
mudskippers. Despite their abundance, studies on the genetic diversity of
mudskippers from Indonesia are limited and there is a noticeable lack of genetic
data in GenBank. This study aims to identify mudskipper species taken from
Bipolo and Oesapa, East Nusa Tenggara, Indonesia using DNA barcoding
targeting the COI gene and to analyze their phylogenetic relationship with other
mudskippers from GenBank. Methods used in this study includes DNA
extraction using Chelex 10% and CTAB method, followed by PCR method with
two sets of primers JgHCO and JgLCO as well as FishF2 and FishR2. Data
analysis was conducted using BLAST and MEGA XI. BLAST analysis revealed
Periophthalmus argentilineatus and Periophthalmodon schlosseri with a
similarity of 99.97% and 90.67% respectively. However, phylogenetic analysis
reveals a clade of initially identified Periophthalmodon schlosseri separated from
other known mudskipper species with a notable 10% genetic distance. This
suggests the presence of a potentially new, undiscovered species yet to be
recorded in GenBank. Further studies incorporating morphometric analysis are
needed to clarify this new potential species and a larger sampling area from a
broader geographic range are necessary to understand genetic diversity of
Mudskippers in Indonesia.
Keywords: mudskipper, DNA barcoding, COI gene, genetic diversity

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Ketersediaan

2005B205

2005 B 2025

Perpustakaan FSM Undip

Tersedia

Informasi Detail

Judul Seri

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No. Panggil

2005 B 2025

Penerbit

: .,

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

NONE

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

-

Info Detail Spesifik

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Pernyataan Tanggungjawab

Rena Galby Andadari

Versi lain/terkait

Lampiran Berkas

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