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Pengaruh Hidrogen Peroksida (H2o2) Pada Tingkat Ketersediaan Air Yang Berbeda Terhadap Pertumbuhan Vegetatif Tanaman Kedelai [Glycine Max (L.) Merr.] Varietas Argomulyo
ABSTRAK
Ika Shintya. 24020115120057. Pengaruh Hidrogen Peroksida (H2O2) pada
Tingkat Ketersediaan Air yang Berbeda terhadap Pertumbuhan Vegetatif
Tanaman Kedelai [Glycine max (L.) Merr.] Varietas Argomulyo. Dibawah
bimbingan Sri Widodo Agung Suedy dan Endah Dwi Hastuti
Tanaman kedelai [Glycine max (L.) Merr.] merupakan salah satu sumber
protein nabati dan komoditas pertanian penting di Indonesi namun
produktivitasnya kurang menentu yang disebabkan oleh berbagai faktor, salah
satunya yaitu ketersediaan air. Ketersediaan air yang rendah di lingkungan
tumbuh dapat menyebabkan tanaman kedelai mengalami cekaman kekeringan.
Pemberian hidrogen peroksida pada tanaman yang mengalami cekaman
kekeringan dalam konsentrasi yang optimum mampu meningkatkan sistem
pertahanan oksidatif tanaman. Penelitian ini bertujuan untuk mengetahui pengaruh
perlakuan pada pertumbuhan vegetatif tanaman kedelai varietas Argomulyo.
Penelitian dilakukan di laboratorium BSF Tumbuhan dan Kebun Percobaan
Departemen Biologi Universitas Diponegoro menggunakan desain pecobaan
rancangan acak lengkap (RAL) faktorial dengan perlakuan 2x4 dan 5 ulangan.
Parameter yang diamati meliputi kandungan klorofil a dan b, lebar pembukaan
stomata daun permukaan atas dan bawah, kadar air relatif daun, tinggi tanaman,
luas daun, bobot kering dan segar tajuk akar, jumlah daun, dan rasio bobot kering
tajuk akar tanaman pada tingkat ketersediaan air 100% dan 35% kapasitas lapang
dengan penyemprotan hidrogen peroksida konsentrasi 0 mM, 0,5 mM, 1 mM, dan
2 mM. Hasil dianalisis ANOVA dan uji DMRT dengan taraf signifikansi 0,05.
Hasil penelitian menunjukkan bahwa penyemprotan hidrogen peroksida tidak
menunjukkan pengaruh berpengaruh terhadap parameter. Tingkat ketersediaan air
berpengaruh terhadap beberapa parameter, dengan hasil menunjukkan
ketersediaan air 100% memiliki nilai lebih tinggi pada tinggi tanaman, jumlah
daun, luas daun, serta bobot segar dan kering tajuk dan akar, sedangkan ratio
bobot tajuk dan akar nilai yang lebih tinggi pada perlakuan ketersediaan air 35%.
Kata kunci : kedelai, hidrogen peroksida, cekaman kekeringan, pertumbuhan,
kapasitas lapang
ABSTRACT
Ika Shintya. 24020115120057. Effect of Hydrogen Peroxide (H2O2) on
Different Water Availability Levels on Vegetative Growth of Soybean
[Glycine max (L.) Merr.] Varieties Argomulyo. Under the guidance of Sri
Widodo Agung Suedy and Endah Dwi Hastuti
The soybean [Glycine max (L.) Merr.] Is one of the important sources of
vegetable protein and agricultural commodities in Indonesia, but its productivity
is uncertain due to various factors, one of which is the availability of water. Low
water availability in the growing environment can cause soybean plants to suffer
from drought stress. Giving hydrogen peroxide to plants experiencing drought
stress in optimum concentration can increase the plant's oxidative defense system.
This study aims to determine the effect of treatment on the vegetative growth of
Argomulyo variety of soybean plants. The study was conducted in the BSF
Laboratory of Plant and Experimental Gardens in the Department of Biology,
Diponegoro University using a factorial completely randomized design (CRD)
design with 2x4 treatment and 5 replications. The parameters observed included
chlorophyll a and b content, stomata opening width of upper and lower surface
leaves, relative water content of leaves, plant height, leaf area, dry weight and
fresh canopy root, number of leaves, and ratio of dry weight of plant root canopy
at the level of availability 100% water and 35% field capacity by spraying
hydrogen peroxide concentrations of 0 mM, 0.5 mM, 1 mM, and 2 mM. The
results were analyzed by ANOVA and DMRT test with a significance level of
0.05. The results showed that spraying hydrogen peroxide did not affect the
parameters. The level of water availability influences several parameters, with the
results showing the availability of water 100% has a higher value in plant height,
number of leaves, leaf area, and fresh and dry weight of crowns and roots, while
the ratio of crown and root weight values are higher in the treatment 35% water
availability. The combination treatment affected several parameters, with the
results showing the most optimal treatment on the relative leaf moisture
parameters with the availability of water 35% and H2O2 0 mM, chlorophyll an on
the treatment of 100% water availability and 2 mM H2O2, chlorophyll b on the
treatment of water availability 100 % and H2O2 0 Mm, width of upper stomata
opening at 100% water availability and 2 mM H2O2, lower stomata opening width
at 100% water availability and 0.5 mM H2O2.
Keywords: soybean, hydrogen peroxide, drought stress, growth, field capacity
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